A new method for efficient detection of Cryptosporidium RNA by real-time reverse transcription-PCR with surfactants
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چکیده
منابع مشابه
A new method for efficient detection of Cryptosporidium RNA by real-time reverse transcription-PCR with surfactants
Cryptosporidium is one of the most common causes of waterborne diseases worldwide. Its oocysts possess a robust wall that is extremely resistant to the chlorine used for potable water disinfection. The current procedures of nucleic acid extraction and purification, such as the freeze–thaw (F/T) method and the commercial kits, are time consuming and expensive. To this end, a surfactant extractio...
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Extraction of high-quality mRNA from Cryptosporidium parvum is a key step in PCR detection of viable oocysts in environmental samples. Current methods for monitoring oocysts are limited to water samples; therefore, the goal of this study was to develop a rapid and sensitive procedure for Cryptosporidium detection in soil samples. The efficiencies of five RNA extraction methods were compared (mR...
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ژورنال
عنوان ژورنال: Water Supply
سال: 2015
ISSN: 1606-9749,1607-0798
DOI: 10.2166/ws.2015.063